Influences of Particles on Pro-inflammatory Signalings in Pre-osteoclasts / 대한정형외과연구학회지
Journal of Korean Orthopaedic Research Society
;
: 18-28, 2006.
Artigo
em Coreano
| WPRIM
| ID: wpr-66467
ABSTRACT
PURPOSE:
To evaluate the roles of macrophages and their influences on the signal transduction in the periprosthetic osteolysis, the pro-inflammatory signals were analyzed in particles-stimulated macrophages. MATERIALS ANDMETHODS:
Raw 264.7 cell line derived from mice macrophages was used as pre-osteoclasts. To increase the stimulatory effects, the particles were composed of PMMA and polyethylene. Under the similar conditions as osteoclast differentiation, we examined the effect of particles on the pro-inflammatory signals in macrophage the production of TNF-alpha, the activity of MAPKs (mitogen-activated phosphorylation kinase), the expression of I-kappaB (Inhibitory (B) and the production of H2O2 and nitric oxide.RESULTS:
The particles stimulated the secretion of TNF-alpha and increased the phosphorylation of p38 and ERK in course of time. The concentration of H2O2 was increased; however the nitric oxide formation was not increased by particle treatment. In addition, the production of H2O2 was synergistically increased by suboptimal stimulation with PMA (phorbol 12-myristate 13-acetate). Expressions of I-kappaB were inhibited by particles.CONCLUSION:
The particles may stimulate the activation of MAPKs, the production of TNF-alpha, reactive oxygen species (ROS) and the activation of NF-kappaB in the pre-osteoclasts. We speculate that particles may mediate the pro-inflammatory signal cascade by the activation of NF-kappaB through ROS in pre-osteoclasts, rather than reactive nitrogen species (RNS). Therefore we suggest that the macrophages in inflammatory osteolysis may have the characteristics of pre-osteoclast as well as pro-inflammatory cell. Further researches should be recommended.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Osteoclastos
/
Osteólise
/
Fosforilação
/
Transdução de Sinais
/
Linhagem Celular
/
NF-kappa B
/
Fator de Necrose Tumoral alfa
/
Espécies Reativas de Oxigênio
/
Polimetil Metacrilato
/
Polietileno
Limite:
Animais
Idioma:
Coreano
Revista:
Journal of Korean Orthopaedic Research Society
Ano de publicação:
2006
Tipo de documento:
Artigo
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