Association of interleukin-6 gene-174G ＞ C polymorphisman with coronary heart disease of Chinese Han population in Nanjing area / 临床检验杂志

ABSTRACT

Objective To investigate the relationship of-174G ＞ C polymorphism of interleukin 6 (IL-6) gene promoter region upstream-with coronary heart disease (CHD) of Chinese Han population in Nanjing area.Methods The polymorphism of IL-6-174G ＞C gene was confirmed in 235 patients with CHD and 175 healthy individuals by PCR-RFLP,and Hardy-Weinberg equilibrium was tested.DNA samples were selected for sequencing to verify their genotype.The concentration of blood sugar,lipid,C reaction protein (CRP) and glycosylated hemoglobin (HbAlc) in the samples were measured simultaneously.Results The genotype distributions of GG,GC and CC were 98.7％,1.3％ and 0 in CHD group and 97.7％,2.3％ and 0 in control group,respectively.The frequencies of G and C alleles were 99.4％,0.6％ and 98.9％,1.1％ in the two groups.There were no statistical significance for frequencies of genotype and alleles between the two groups (all P ＞ 0.05).Compared with the control group,the differences of smoking,systolic blood pressure (SBP),diastohc blood pressure (DBP),triglyceride (TG),cholesterol (CHOL),low-density lipoproteins-cholesterol (LDL-C),high-density lipoproteins-cholesterol (HDL-C),apolipoprotein A-I (ApoA-I),ApoB,CRP and HbA1 c were statistically significant (all P ＜ 0.05),while age,sex and blood glucose were not statistically significant (all P ＞ 0.05).Conclusion IL-6-174 G ＞ C gene polymorphism should not remarkably correlated with CHD in Chinese Han population in Nanjing area.