Changes of the fungal microbiota in inflamed intestinal mucosa of the patients with Crohn's disease / 肠外与肠内营养

ABSTRACT

Objective:The study aimed to characterize the difference of the fungal microbiota between the inflamed and non-inflamed intestinal mucosa in patients with Crohn's disease (CD) and explore the potential relation between their changes and the pathogenesis of CD.Methods:Seven patients with active CD were enrolled and the specimens was obtained from the inflamed and non-inflamed mucos a during operation.Tissue DNA was extracted and then amplified by nested polymerase chain reaction (PCR) with the premier sets NS 1/FR1 and EF390/GC-FR1.Denaturing gradient gel electrophoresis was subsequently conducted to profile the structure and composition of the fungal microbiota.Results:Compared to those of noninflamed region,the species richness and diversity of the fungal microbiota in inflamed region were significantly increased (P ＜ 0.05).The predominant fungal composition in inflamed region was significantly altered,mainly characterized by the increase of opportunistic pathogens including Candida albicans,Candida tropicalis,Gibberella moniliformis,Alternaria brassicicola and Cryptococcus neoformans relative to that of noninflamed region (P ＜ 0.05),compared with those of noninflamed region.Conversely,the proportion of the commensals,for example Saccharomyces cerevisiae,Saccharomy,ces castellii,Penicillum chrysogenum and Laccaria bicolor,were significantly decreased in inflamed region (P ＜ 0.05).Conclusion:The fungal microbiota of the inflamed intestinal mucosa is severely dysbiotic in CD patients.The colonization of some pathogenic fungi could participate and result in inflammatory damage.