Regulatory Effect of Liuwei Dihuang Pills on Connexin 43 Expression in Hepatocarcinoma CBRH7919 Cells in Vitro / 广州中医药大学学报
Journal of Guangzhou University of Traditional Chinese Medicine
;
(6): 872-877, 2017.
Artigo
em Chinês
| WPRIM
| ID: wpr-666859
ABSTRACT
Objective To investigate the effect of Liuwei Dihuang Pills (LDP)on the expression of connexin 43 (Cx43) in rat hepatocarcinoma CBRH7919 cells. Methods LDP-containing serum was prepared by serum pharmacological method. The protein expression of Cx43 in CBRH7919 cells was determined by Western blotting method, indirect immuno-fluorescent staining labeled by fluorescein isothiocyanate (FITC)-laser scanning confocal microscope technology and flow cytometry. And the mRNA expression of Cx43 in the hepatoma cells was detected by reverse transcription real-time quantitative polymerase chain reaction(RT-qPCR). Results Compared with the blank serum control group,the protein and mRNA expression levels of Cx43 were increased by volume fraction of 2.5%, 5%, 10% LDP-containing serum (P < 0.05 or P < 0.01). Conclusion LDP play an anti-cancer role through promoting the expression of Cx43 in CBRH7919 cells, altering Cx43 membrane location, and improving the gap junctional intercellular communication(GJIC).
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DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Idioma:
Chinês
Revista:
Journal of Guangzhou University of Traditional Chinese Medicine
Ano de publicação:
2017
Tipo de documento:
Artigo
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