The clinnical significance of TRPM8 ion channels over-expressed in pancreatic cancer / 中华肝胆外科杂志

ABSTRACT

Objective TRPM8 as a clinical biomarker and potential drug target may provide a guidance in accurately judging the malignant potential and prognosis of pancreatic cancer.Downregulating the expression of TRPM8 leads to inhibition of the proliferation of pancreatic cancer cells and this may provide a new target for personalized pancreatic cancer treatment.Methods The pancreatic cancer tissues of 77 patients and the normal pancreas tissues of 30 persons were collected and examined by immunohistochemistry.PCR,gene knockout,Western Blot,CCK8 and patch clamp experiments were performed on pancreatic cancer cell lines BxPC-3,PANC1 and normal pancreatic cell line HPCY-5.Results Immunohistochemistry and Western Blot experiments showed that the TRPM8 channel protein was highly expressed in cancer cells,and the high expression of channel proteins was related to the degree of tumor differentiation (P ＜ 0.05).The detection of the TRPM8 mRNA in pancreatic cancer cell lines BxPC-3 and PANC1 by QPCR showed absolutely high expressions relative to the normal cell line HPCY-5.The results of patch clamp and CCK8 showed that the membrane currents and proliferation of tumor cells were significantly decreased after TRPM8 gene knockout,and the difference is significant (P ＜ 0.05).Conclusions The TRPM8 ion channel was higher in pancreatic cancer cells than that in normal pancreatic cells.Overexpression of TRPM8 was closely related to tumor cell proliferation and invasion ability.