Identification of Differentially Expressed Genes from Serum Deprived p388D1 Cells
Korean Journal of Pathology
;
: 488-493, 1998.
Artigo
em Coreano
| WPRIM
| ID: wpr-66758
ABSTRACT
This experiment is designed to find differentially expressed genes in p388D1 cells that are specific for the serum deprived state. Serum starvation induces cells to enter the quiscent state in the cell cycle and is used to arrest cell growth or synchronize the cell cycle. Differential display and ribonuclease protection assay were used to identify quantitative change in gene expression. Nineteen genes that showed a differential expression in the differential display were cloned and 7 clones were verified by a ribonuclease protection assay. Among the 7 clones clone-16 showed same expression pattern in comparison with the differential display. Deduced amino acid sequences of clone-16 had N-glycosylation motif and seems to be a secretory protein. Getting a full sequence of clone-16 is critical for the characterization of it.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Ribonucleases
/
Inanição
/
Expressão Gênica
/
Ciclo Celular
/
Sequência de Aminoácidos
/
Células Clonais
Tipo de estudo:
Estudo diagnóstico
Idioma:
Coreano
Revista:
Korean Journal of Pathology
Ano de publicação:
1998
Tipo de documento:
Artigo
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