Ophiopogonin-B inhibited cell mitosis by Myt / Cdc2 pathway in H460 cells / 中国药理学通报

ABSTRACT

Aim To explore the effects of ophiopogo-nin-B (OP-B)on cell cycle and mitosis in non-small cell lung cancer (NSCLC)H460 cells in vitro and the underlying mechanisms. Methods TUNEL immuno-histochemical assay was used to detect the change of the nuclear matter. DAPI staining was used to detect the change of the nuclear morphology and the mitosis status. Meanwhile,Western blot was performed to de-termine the protein level of the proteins regulating cell cycle and mitosis. Results OP-B significantly arres-ted cell cycle in G0 / G1 phase and inhibited the mitosis in H460 cells at the concentration of 10 μmol·L - 1 . Meanwhile,it inhibited the protein level of cyclinD1, cyclinB1,and up-regulated the expression of Myt and the phosphorylation level of Cdc2. Conclusion OP-B inhibits cell mitosis in A549 cells through Myt/ Cdc2 signaling pathway.