Tetracycline hydrochloride promotes rat bone marrow mesenchymal stem cells proliferation in vitro / 中国组织工程研究

ABSTRACT

BACKGROUND: Tetracycline hydrochloride is shown to promote viability and proliferation of human embryonic osteoblasts cultured in vitro, increase the production of type I collage and osteocalcin. OBJECTIVE: To study the proliferation activity of bone marrow mesenchymal stem cells (BMSCs) cultured by tetracycline hydrochloride in vitro. METHODS: Passage 3 BMSCs from Sprague-Dawley rats were cultured and divided into two groups cells in control group were cultured in complete medium containing fetal bovine serum, and those in experimental group were cultured in complete medium containing tetracycline hydrochloride. After days 1-8 of culture, MTT and cell counting assay were used to detect cell viability and proliferation in the two groups. RESULTS AND CONCLUSION: (1) Cell counting assay with time, the number of cells in both groups was increased, but the cell number in the experimental group was higher than that in the control group at days 4-8 of culture (P < 0.05). (2) MTT assay With time, the number of cells in both groups was increased incubation period was at day 1, logarithmic growth period was within days 3-6, and plateau period began at day 7. Then, the cells continued to proliferate, and the cell proliferation was higher in the experimental group than the control group at days 5-8 of culture (P < 0.05). To conclude, tetracycline hydrochloride can significantly enhance BMSCs proliferation.