Butein promotes the role of mH2A in MAPK signaling pathway through targeting GRP78 in regulating biological behaviors of melanoma / 中南大学学报(医学版)

ABSTRACT

Objective:To investigate the effect of mH2A and Butein on mitogen-activation protein kinase (MAPK) signaling pathway through targeting glucose regulated protein of 78 (GRP78) in regulating the biological behavior of melanoma.Methods:Immunohistochemistry was used to detect the expressions of mH2A and GRP78 in melanoma and adjacent normal tissues.The relationship between mH2A and GRP78,and the dinicopathological data was analyzed.The relationship between GRP78 and mH2A protein was detected by immunoprecipitation assay.The protein expressions of mH2A and GRP78 was detected by Western blot.The invasion ability of melanoma after sliencing mH2A was detected by Transwell invasion assay.The migration ability of melanoma after sliencing mH2A was detected by wound healing assays.The protein expressions of MEK and ERK1 after sliencing mH2A was detected by Westem blot.Results:The expressions of mH2A and GRP78 in melanoma tissues was significantly lower than that in adjacent normal tissues (P＜0.05).Butein enhanced the expression of mH2A (P＜0.05).The mH2A regulated the GRP78 protein (P＜0.05).Silencing mH2A promoted the invasion and migration of melanoma A375 cells.The MEK and ERK1 protein expressions were up-regulated after silencing Butein.Conclusion:Butein promotes the role of mH2A in regulating GRP78 and modulating the biological behavior of melanoma cells through MAPK signaling pathway.