Content Comparison of Flavonoids in Tribulus Terrestris from Different Habitats / 中国药师

ABSTRACT

Objective:To establish an HPLC method for the determination of rutin, luteolin, quercetin, kaempferol, isorhamnetin and chrysin in Tribulus terrestris L. Methods:The chromatographic column was Welch Ultimate LP-C18(250 mm ×4.6 mm, 5 μm), the mobile phase was acetonitrile and 0. 05% phosphoric acid with gradient elution,the detection wavelength was 360 nm,and the flow rate was 1. 0 ml·min-1 . Results: The linear relationship of standard curve of rutin, luteolin, quercetin, kaempferol, isorhamnetin and chrysin was good (r≥0. 999 4). The average recovery was 97. 5%-99. 7% with RSD≤1. 24% (n=6). Conclusion:The meth-od is simple, and the precision,recovery and repeatability all meet the requirements,which can be used for the content determination of Tribulus terrestris L.