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Determination of Content and Entrapment Efficiency of VEC-5 Liposomes by HPLC / 中国药师
China Pharmacist ; (12): 1642-1645, 2015.
Artigo em Chinês | WPRIM | ID: wpr-670077
ABSTRACT

Objective:

To establish an HPLC method for the determination of content and entrapment efficiency of HIV-1 virus in-fection factor Vif inhibitor VEC-5 liposomes.

Methods:

VEC-5 liposomes were prepared by a method of freeze-drying and reconstruc-tion. The separation of free drug from the liposomes was achieved by ultracentrifugation, and an HPLC method was used to determine the content and entrapment efficiency of VEC-5 liposomes.

Results:

The linear range of VEC-5 was 20-100 μg·ml-1(r=0. 999 0). The average recovery was 100. 25% and RSD was 0. 93%(n=9). The content of three batches of VEC-5 liposomes was 98. 63%, 100. 43% and 102. 65%, respectively within the range of 90%-110%, and the entrapment efficiency was 94. 89%, 93. 68% and 94. 56%, respectively, which was above 90%.

Conclusion:

The method is accurate and reliable, which can be used to determine the content and entrapment efficiency of VEC-5 liposomes.

Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Idioma: Chinês Revista: China Pharmacist Ano de publicação: 2015 Tipo de documento: Artigo

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Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Idioma: Chinês Revista: China Pharmacist Ano de publicação: 2015 Tipo de documento: Artigo