Determination of Content and Entrapment Efficiency of VEC-5 Liposomes by HPLC / 中国药师
China Pharmacist
;
(12): 1642-1645, 2015.
Artigo
em Chinês
| WPRIM
| ID: wpr-670077
ABSTRACT
Objective:
To establish an HPLC method for the determination of content and entrapment efficiency of HIV-1 virus in-fection factor Vif inhibitor VEC-5 liposomes.Methods:
VEC-5 liposomes were prepared by a method of freeze-drying and reconstruc-tion. The separation of free drug from the liposomes was achieved by ultracentrifugation, and an HPLC method was used to determine the content and entrapment efficiency of VEC-5 liposomes.Results:
The linear range of VEC-5 was 20-100 μg·ml-1(r=0. 999 0). The average recovery was 100. 25% and RSD was 0. 93%(n=9). The content of three batches of VEC-5 liposomes was 98. 63%, 100. 43% and 102. 65%, respectively within the range of 90%-110%, and the entrapment efficiency was 94. 89%, 93. 68% and 94. 56%, respectively, which was above 90%.Conclusion:
The method is accurate and reliable, which can be used to determine the content and entrapment efficiency of VEC-5 liposomes.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Idioma:
Chinês
Revista:
China Pharmacist
Ano de publicação:
2015
Tipo de documento:
Artigo
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