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A preliminary study of injectable tissue-engineered bone / 实用口腔医学杂志
Journal of Practical Stomatology ; (6)1995.
Artigo em Chinês | WPRIM | ID: wpr-670489
ABSTRACT

Objective:

To develop injectable tissue-engineered bone.

Methods:

Bone marrow cells isolated from lilac bone of New Zealand rabbits were cultured and induced to differentiate Into osteoblasts. The osteoblasts were mixed with 20 g/L alginate sodium solution to generate osteoblasts/alginate composite with final cellular density of 5 ? 10 6/ml. Calcium chloride was used as cross-linking agent. The osteoblasts/alginate composite was injected into the dorsal subcutaneous tissue of nude mice. The injected material with surounding tissue were examined with X-ray and histopathologic technique.

Results:

Four and eight weeks after injection, the hard knobbles were easily palpated under the dorsal skin of the animals. On X-ray photograph the knobbles showed calcified tissue image. In histological analysis, new bone formation was observed in the osteoblasts/alginate composite. The osteogenesis was in association with regenerated hematopoietic bone marrow.

Conclusion:

New bone tissue can be created through the injection of alginate sodium mixed with marrow stromal osteoblasts.

Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Idioma: Chinês Revista: Journal of Practical Stomatology Ano de publicação: 1995 Tipo de documento: Artigo

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Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Idioma: Chinês Revista: Journal of Practical Stomatology Ano de publicação: 1995 Tipo de documento: Artigo