A preliminary study of injectable tissue-engineered bone / 实用口腔医学杂志
Journal of Practical Stomatology
;
(6)1995.
Artigo
em Chinês
| WPRIM
| ID: wpr-670489
ABSTRACT
Objective:
To develop injectable tissue-engineered bone.Methods:
Bone marrow cells isolated from lilac bone of New Zealand rabbits were cultured and induced to differentiate Into osteoblasts. The osteoblasts were mixed with 20 g/L alginate sodium solution to generate osteoblasts/alginate composite with final cellular density of 5 ? 10 6/ml. Calcium chloride was used as cross-linking agent. The osteoblasts/alginate composite was injected into the dorsal subcutaneous tissue of nude mice. The injected material with surounding tissue were examined with X-ray and histopathologic technique.Results:
Four and eight weeks after injection, the hard knobbles were easily palpated under the dorsal skin of the animals. On X-ray photograph the knobbles showed calcified tissue image. In histological analysis, new bone formation was observed in the osteoblasts/alginate composite. The osteogenesis was in association with regenerated hematopoietic bone marrow.Conclusion:
New bone tissue can be created through the injection of alginate sodium mixed with marrow stromal osteoblasts.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Idioma:
Chinês
Revista:
Journal of Practical Stomatology
Ano de publicação:
1995
Tipo de documento:
Artigo
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