A preliminary study of injectable tissue-engineered bone / 实用口腔医学杂志

ABSTRACT

Objective: To develop injectable tissue-engineered bone. Methods: Bone marrow cells isolated from lilac bone of New Zealand rabbits were cultured and induced to differentiate Into osteoblasts. The osteoblasts were mixed with 20 g/L alginate sodium solution to generate osteoblasts/alginate composite with final cellular density of 5 ? 10 6/ml. Calcium chloride was used as cross-linking agent. The osteoblasts/alginate composite was injected into the dorsal subcutaneous tissue of nude mice. The injected material with surounding tissue were examined with X-ray and histopathologic technique. Results: Four and eight weeks after injection, the hard knobbles were easily palpated under the dorsal skin of the animals. On X-ray photograph the knobbles showed calcified tissue image. In histological analysis, new bone formation was observed in the osteoblasts/alginate composite. The osteogenesis was in association with regenerated hematopoietic bone marrow. Conclusion: New bone tissue can be created through the injection of alginate sodium mixed with marrow stromal osteoblasts.