Tissue engineering of bone and cartilage composite / 实用口腔医学杂志

ABSTRACT

砄bjective To fabricate tissue engineered bone cartilage composite. Method: Rabbit marrow stem cells (MSCs) were in vitro cultured, expanded and induced to differeciate to osteoblasts. Chondrocytes were obtained by collagenase type Ⅱ digestion of rabbit ear cartilage. Osteoblasts and chondrocytes were co seeded into different part of natural coral scaffold, and then implanted subcutaneously into the back of nude mice. Two months after implantation,the specimens were harvested and bone cartilage composites formation was observed by gross inspection and histologic observation. Results: The newly formed tissue was composed of two parts. One part was glisteringly white and another part was dark red. There was an obvious boundary between the two parts. Microscopic observation revealed successful restoration of bone cartilage composite. Conclusion:Bone cartilage composite can be prepared by co deeding of osteoblasts and chondrocytes into natural coral scaffold.