The therapeutic mechanism of adenovirus mediated CD/5-FC system in the treatment of oral squamous carcinoma cells / 实用口腔医学杂志

ABSTRACT

Objective: To study the therapeutic mechanism of adenovirus mediated cytosine deaminase /5-fluorocytosine (AdCMVCD/ 5-FC) suicide gene system in the treatment of oral squamous carcinoma cells in vitro. Methods: 3H-thymidine ( 3H-TdR) incorporation assay, flow cytometry (FCM), transmission electron microscope and TUNEL (TdT-mediated dUTP Nick End Labeling)assay were used to detected the changes of Tca8113 cells after the treatment with AdCMVCD/5-FC system. Results: After treatment with CD/5-FC system, 3H-TdR incorporation rate of the cells decreased and significantly decreased between different MOI (multiple of infection) at 5-FC 10 -3 mol/L (P0.05); many apoptotic cells were found under transmission electron microscope and the positive rate of apoptotic cells increased from (4.40?0.87)% (before treatment) to (15.80?1.55)% (P