Detection of Streptococcus sobrinus by polymerase chain reaction / 实用口腔医学杂志
Journal of Practical Stomatology
;
(6)2000.
Artigo
em Chinês
| WPRIM
| ID: wpr-670649
ABSTRACT
Objective:
To develop a PCR method for detecting S treptococcus sobrinus (S.s) in conventional culture.Method:
A pair of specific primers were designed from the dexA gene of S.s , the genome DNA of 12 strains of Mutans Streptococci (8 serotypes from a ~h) and 23 species of the bacteria which were commonly found in oral cavity wer e tested by PCR amplification, the PCR products were identified by electrophore sis.Result:
Only S.s of serotype d and g could produ ce 277 bp DNA fragments, and the PCR method could detect less than 1 000 copies of S.s.Conclusion:
PCR method is specific and sen sitive in the detection of S.s.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Tipo de estudo:
Estudo diagnóstico
Idioma:
Chinês
Revista:
Journal of Practical Stomatology
Ano de publicação:
2000
Tipo de documento:
Artigo
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