Construction of mcpr1gene vector and expression of mcpr1 in escherichia coli / 实用口腔医学杂志
Journal of Practical Stomatology
;
(6)2000.
Artigo
em Chinês
| WPRIM
| ID: wpr-670706
ABSTRACT
Objective:
To construct mcpr1prokaryoti c expression vector and to express MCPR1 protein.Methods:
PCR was used to obtain coding region of mcpr1. Construction of a high-level fusion protein expression vector pGEX-4T-mcpr1 was conducted by inserting the fra gment of coding region of mcpr1into a fusion protein expression vector pGEX -4T-1. Then the recombinant plasmid was transferred into E. colito prepar e the MCPR1/GST fusion protein. DNA sequencing and endonucleases digesting were used to check the coding region.Results:
pGEX-4T- mcpr1 wa s constructed successfully and the coding region was inserted into the vector co rrectly. A new protein band of 36 000 was observed by SDS-PAGE analysis after i nduction by IPTG. The 36 000 protein amounted to 39 percent of the total prote in and existed mostly in precipitation of broken bacteria.Conclusion:
MCPR1 protein can be expressed in E. coliexpression system and purif ied initially.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Idioma:
Chinês
Revista:
Journal of Practical Stomatology
Ano de publicação:
2000
Tipo de documento:
Artigo
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