The clinical evaluation of the expression of the PCNA and cell nuclear area in the different layers of oral dysplasia epithelium / 实用口腔医学杂志

ABSTRACT

Objective: To study the expression of the proliferating c el l nuclear antigen (PCNA) and cell nuclear area in the different layers of oral d ysplasia epithelium. Methods: S-P immunohistochemical method an d morphometric technique(computer image measurement method) were used to examine the PCNA and cell nuclear area in the different layers of the normal oral epith elium(NOR) in 8 cases and abnormal oral epithelium in 46 cases including mild d ysplasia(LD), moderate dysplasia(MD), severe dysplasia(SD) and well-differentia ted squamous cell carcinoma(SCC). Results: The positive PCNA sta ining cells in normal epithelium was found mainly in the basal layer. The prolif erating index(PI) in the whole layer of abnormal oral epithelium were increased (P05). PI in the basal la yer of abnormal epithelium was higher than in NOR (P0.05). PI in the spi nosum layer and superficial layer (granulosum and lucidum layer) increased with the development of the lesions, The cell nuclear area in the basal and spinosum layer of the abnormal epithelium increased(P