Effects of estradiol on the proliferation and metastasis of adenoid cystic carcinoma SAcc83 cells / 实用口腔医学杂志

ABSTRACT

Objective:To investigate the effects of 17-? estradiol (E2) on the proliferation and metastasis of adenoid cystic carcinoma SAcc83 cells.Methods:The effects of E2 on the proliferation of SAcc83 cells were studied by MTT assay and flow cytometry.The invasion potential of the cells was investigated with chicken embryo heart invasion assay.The in vivo growth and metastasis of SAcc83 cell induced tumor were studied in ovary-resected female nude mice. Results:E2 at 10 -7 ,10 -8 and 10 -9 mol/L promoted the proliferation of SAcc83 cells. E2 at 10 -7 mol/L showed the strongest growth stimulation effects and increased the cell proliferation by 34.4%.With exposure time of 1,2 and 3 d it increased the proliferation index by 33.3%,40.9% and 17.8% respectively;with the exposure time of 12,18 and 24 h,it increased S phase cells in cell cycle by 12.3%,3.3% and 9.7% respectively. Neoplasm developed in all nude mice after subcutaneous transplantation of 106 cells.In the mice treated with 0.2 ml of E2 at 9.55 ?10 -5 mol/L every day for 15 days neoplasm volume doubling time was 61.5 h,that in control mice 76.5 h.In lung metastasis trial, 42 days after tail injection of the cells the metastasis foci in E2 treated mice inreased by 41.2%.Conclusion:E2 may increase the proliferation and metastasis of adenoid cystic carcinoma cells.