Preliminary study of the cultivation and growth characteristics of human salivary gland epithelial cells / 实用口腔医学杂志

ABSTRACT

Objective:To establish a culture method of human salivary gland epithelial cells and to study their growth characteristics in vitro.Methods:Tissue explant technic was employed to culture human salivary gland epithelial cells in serum free medium (SFM),1∶1 DMEM/F12 and 1∶1 DMEM/F12 containing 25 ml/L fetal boven serum (FBS) respectively.The morphology of the cultured cells was observed by phase contract microscope.The cell growth was studied by cell counting.The cells were identified by HE staining,PAS staining and SP staining.Results:Growth of human salivary gland epithelial cells was observed in primary culture in the three kinds of medium in all 10 cases. The cultured cells were epidermoid, positive for cytokeratin, negative for Vimentin and positive for PAS staining. The cells in SFM could be subcultured for five passages,while only for one passage in the other two kinds of medium.Conclusion:SFM is superior to serum free 1∶1 DMEM/F12 or 1∶1 DMEM/F12 containing 25 ml/L FBS for the culture of human salivary gland epithelial cells.