Effects of berberine hydrochloride on human periodontal ligament cells cultured in vitro / 实用口腔医学杂志

ABSTRACT

Objective:To explore the effects of berberine hydrochloride on human periodontal ligament cells(HPDLCs) cultured in vitro. Methods:Cell proliferation activity was assayed by using MTT method. The total protein was detected by Coumassie Bright Blue method and the alkaline phosphatase (ALP) activities were measured by spectrophotometric assay. Results:①Comparing with the control group, the proliferative ability of PDLCs in berberine hydrochloride (0.005,0.010,0.020,0.030 g/L)groups at 24 h and (0.005,0.010,0.020 g/L)groups at 48 h and (0.010,0.020 g/L)groups at 72 h were considerably increased(P