Interaction and Molecular Simulation between Gossypol and Human Serum Albumin / 中国药师
China Pharmacist
;
(12): 881-883,887, 2015.
Artigo
em Chinês
| WPRIM
| ID: wpr-671115
ABSTRACT
Objective:
To explore the interaction between gossypol and human serum albumin ( HSA) .Methods:
The interaction of gossypol and HSA under physiological conditions was studied by fluorescence spectroscopy, and the molecular docking software was used to simulate the interaction.Results:
The binding constant of gossypol and HSA at 293K and 303K was 2. 390 6 × 105 L·mol-1 and 3. 576 8 × 103 L·mol-1 , respectively. There was one binding site on HSA for gossypol. Hydrogen bond and Van Der Waals inter-actions were involved in the binding process. The binding of gossypol and HAS was closer to tyrosine residue in HSA. The molecular simulation analysis verified the above results.Conclusion:
The gossypol-induced fluorescence quenching of HSA belongs to a static quenching procedure.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Idioma:
Chinês
Revista:
China Pharmacist
Ano de publicação:
2015
Tipo de documento:
Artigo
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