Quantification of tolvaptan in rabbit plasma by LC-MS/MS:Application to a pharmacokinetic study / 药物分析学报
Journal of Pharmaceutical Analysis
;
(6): 371-377, 2015.
Artigo
em Chinês
| WPRIM
| ID: wpr-672244
ABSTRACT
A sensitive, selective and high-throughput liquid chromatography–tandem mass spectrometry (LC–ESI–MS/MS) method was developed and validated for the quantitation of tolvaptan in rabbit plasma. Sample clean-up involved liquid–liquid extraction (LLE) and chromatography was performed on Zorbax SB C18 analytical column (50 mm ? 2.1 mm, 3.5 mm) using 0.1%formic acidmethanol (2080, v/v) as the mobile phase. The parent-product ion transitions for the drug (m/z 449.2-252.1) and IS (m/z 456.2-259.2) were monitored on a triple quadrupole mass spectrometer, operating in the multiple reaction monitoring (MRM) and positive ion mode. The method was validated over the concentration range of 0.10–1000.00 ng/mL and successfully applied to a pharmacokinetic study of healthy rabbits.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Idioma:
Chinês
Revista:
Journal of Pharmaceutical Analysis
Ano de publicação:
2015
Tipo de documento:
Artigo
Similares
MEDLINE
...
LILACS
LIS