Phytochemical analysis and antagonistic activity of Ixora macrothyrsa on multidrug resistant bacteria

ABSTRACT

Objective: The present investigation was an attempt to identify the potent drug principles ofIxora macrothyrsa flower against Methicillin Resistant Staphylococcus aureus and Acinetobacter baumannii. Methods: Hot extraction soxhlet method was performed for the extraction of Ixora macrothyrsa flower powder with different solvents. Antagonistic activity checked for all extracts with MTCC standard pathogenic strains and multiple drug resistant strains. Followed this minimal inhibitory concentration, phenol estimation, High performance thin layer chromatography analysis and Gas chromatography- mass spectrometry analysis were performed for Ixora macrothyrsa ethanolic extract. Results: The results from the present study indicate that the flowers of the plant has rich source of phytochemicals in ethanol extracts compare to acetone and methanol extracts. The quantitative estimation of ethanolic extracts of Ixora macrothyrsa showed phenol content 83.67±.04 μg/g of ascorbic acid equivalent. The ethanol extract exhibited good antibacterial activity against all tested pathogens. In this study we point out that ethanol and methanol extracts showed supreme activity against Methicillin Resistant Staphylococcus aureus (MRSA) and Acinetobacter baumannii. The MIC of Ixora macrothyrsa ethanolic extract was identified as 22μg/ml for MRSA and 200μg/ml for Acinetobacter baumannii. The HPTLC analysis of the ethanol plant extract inferred that it contain both polyphenol and terpenoids. In GC-MS analysis the major constituent was identified as Ethene (2-chloroethoxy)-, 1-propanol 2-chloro, urethane with 16.39 area %. Conclusions: Hence it is inferred from our study that the plant flower would be a promising source of phytomedicine against multidrug resistant strains MRSA and Acinetobacter baumannii.