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Cytotoxicity and genotoxicity assessment of Euphorbia hirta in MCF-7 cell line model using comet assay
Article em Zh | WPRIM | ID: wpr-672685
Biblioteca responsável: WPRO
ABSTRACT
Objective:To evaluate the cytotoxicity and genotoxicity activity of Euphorbia hirta (E. hirta) in MCF-7 cell line model using comet assay. Methods: The cytotoxicity of E. hirta extract was investigated by employing brine shrimp lethality assay and the genotoxicity of E. hirta was assessed by using Comet assay. Results: Both toxicity tests exhibited significant toxicity result. In the comet assay, the E. hirta extract exhibited genotoxicity effects against MCF-7 DNA in a time-dependent manner by increasing mean percentage of DNA damage. The extract of E. hirta showed significant toxicity against brine shrimp with an LC50 value of 620.382 μg/mL (24 h). Comparison with positive control potassium dichromate signifies that cytotoxicity exhibited by the methanol extract might have moderate activity. Conclusion:The present work confirmed the cytotoxicity and genotoxicity of E. hirta. However, the observed toxicity of E. hirta extracts needs to be confirmed in additional studies.
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Texto completo: 1 Índice: WPRIM Idioma: Zh Revista: Asian Pacific Journal of Tropical Biomedicine Ano de publicação: 2013 Tipo de documento: Article
Texto completo: 1 Índice: WPRIM Idioma: Zh Revista: Asian Pacific Journal of Tropical Biomedicine Ano de publicação: 2013 Tipo de documento: Article