Evaluation of anti-bacterial and anti-oxidant potential of andrographolide and echiodinin isolated from callus culture of Andrographis paniculata Nees
Asian Pacific Journal of Tropical Biomedicine
;
(12): 604-610, 2013.
Artigo
em Chinês
| WPRIM
| ID: wpr-672746
ABSTRACT
Objective:
To evaluate the anti-bacterial and anti-oxidant activity of andrographolide (AND) and echiodinin (ECH) of Andrographis paniculata.Methods:
In this study, an attempt has been made to demonstrate the anti-microbial and anti-oxidant activity of isolated AND and ECH by broth micro-dilution method and 2,2-diphenyl-2-picryl-hydrazyl (DPPH) assay, respectively. Structure elucidation was determined by electro-spray ionization-MSD, NMR (1H and 13C) and IR spectra.Results:
AND was effective against most of the strains tested including Mycobacteriumsmegmatis, showing broad spectrum of growth inhibition activity with Minimum inhibitory concentration values against Staphylococcus aureus (100 μg/mL), Streptococcus thermophilus (350 μg/mL) Bacillus subtilis (100 μg/mL), Escherichia coli (50 μg/mL), Mycobacterium smegmatis (200 μg/mL), Klebsiella pneumonia (100 μg/mL), and Pseudomonas aeruginosa (200 μg/mL). ECH showed specific anti-bacterial activity against Staphylococcus aureus, Escherichia coli, Bacillus subtilis and Pseudomonas aeruginosa at a concentration higher than 225 μg/mL. Both AND and ECH were not effective against the two yeast strains, Candida albicans and Saccharomyces cerevisiae tested in this study.Conclusion:
This preliminary study showed promising anti-bacterial activity and moderate free radical scavenging activity of AND and ECH, and it may provide the scientific rationale for its popular folklore medicines.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Idioma:
Chinês
Revista:
Asian Pacific Journal of Tropical Biomedicine
Ano de publicação:
2013
Tipo de documento:
Artigo
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