THE RAPID DETERMINATION OF ABO BLOOD GROUP IN BODY FLUIDS (OR STAINS)BY DOT-ELISA USING ENZYME-LABELLED MONOCLONAL ANTIBODY / 中国法医学杂志
Chinese Journal of Forensic Medicine
;
(6)1987.
Artigo
em Chinês
| WPRIM
| ID: wpr-673123
ABSTRACT
One step dot-ELISA method for the rapid determination of ABO blood groups in humam body fluids(or stains)was established using enzyme-labelled anti-A,--B and anti--H monoclonal antibody (McAb).The sample was applied on the nitrocellulose membrance as a dot. After washing, the appropriate McAb wasadded on top of the dot, and then followed by adding 3, 3'-diaminobenzidine(DAB). The brown color indi-cated the positive reaction. The ABO blood typing of 521 saliva samples including both secretor and non-se-cretor were carried out. All the results were correct. The advantages of this method are accurate, sensitive,rapid, easy to perform, as well as not time consuming. It is more sensitive than the conventional hemaggluti-naton inhibition test.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Idioma:
Chinês
Revista:
Chinese Journal of Forensic Medicine
Ano de publicação:
1987
Tipo de documento:
Artigo
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