Experimental study of posterior vitreous detachment with plasmin in human eyes / 中华眼底病杂志
Chinese Journal of Ocular Fundus Diseases
;
(6)1996.
Artigo
em Chinês
| WPRIM
| ID: wpr-673619
ABSTRACT
Objective To investigate the dosage, efficacy and safety of intravitreal injection of plasmin in producing posterior vitreous detachment (PVD), and the possible role of plasmin in degrading adhesion glycoproteins of inner limiting membrane (ILM). Methods Twenty eyes of young human cadavers within 24 hours after death were divided into 4 groups that received 0 1 ml balanced salt solution (group 1) as control, 1 (group 2), 2 (group 3), or 3 (group 4) U of human plasmin. Optical and transmission electron microcopies were performed to examine the ultrastructure of the vitreoretinal interface. Electron immunocytochemical techniques were carried out on ILM to estimate the content of fibronectin (FN) and laminin (LN). Flow cytometry was used for cell viability analysis of variance (ANOVA) and Tukey test was employed for statistical analysis. Results Microscopy demonstrated that plasmin especially in group 4 cleaved the attachment of the vitreous collagen fibrils to the ILM with no evident damage to the inner retina. The content of LN, FN in ILM decreased with injection of plasmin (group 3 and 4 had statistical significance from control group for FN, P
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Idioma:
Chinês
Revista:
Chinese Journal of Ocular Fundus Diseases
Ano de publicação:
1996
Tipo de documento:
Artigo
Similares
MEDLINE
...
LILACS
LIS