Study on the perinatal Chlamydia Trachomatis infection in Chongqing area using urine Gap-LCR-ELISA / 中华围产医学杂志

ABSTRACT

Objective To study the prevalence of perinatal chlamydia trachomatis infection in the city of Chongqing. Methods First void urine (FVU) samples and cervical smear from 512 pregnant women (gestational age≥28 weeks) were collected. According to the “expanded gold standard”, the methodologic indices were compared between Gap LCR ELISA using plasmid probes and omp1 probes on FVU and cervical smear, respectively. Results (1) CT infection in pregnancy is usually asymptomatic. Forty two CT positive cases were confirmed by “expanded gold standard” indicating a prevalence of CT infection in pregnant women in Chongqing was 8.20%(42/512). Among these 42 women, 37 (88.1%) were detected from both FVU and cervical smear, while positive results were shown in 4 cases (9.5%) from cervical smear and only one case (1/42) 2.4% from FVU . (2) The sensitivity of Gap LCR ELISA using plasmid probes and ompl probes were 90.48% and 71.43% ( P 0.05). The specificity of all Gap LCR ELISA tests were 100%. Conclusions FVU plasmid Gap LCR ELISA is a noninvasive, highly sensitive and specific method which is suitable for large scale screening for perinatal CT infections in pregnant women in developing countries and regions.