Simultaneous Determination of Three Components of Puma Nose Drops by RP-HPLC / 中国药房
China Pharmacy
;
(12)1991.
Artigo
em Chinês
| WPRIM
| ID: wpr-673993
ABSTRACT
OBJECTIVE:
To establish a RP-HPLC method for simultaneous determination of chlorapheniramine maleate,furacilin and ephedrine hydrochloride in Puma nose drops.METHODS:
The analysis was carried on a XDB C 8 column;the mo?bile phase was composed of methanol(A),acetonitrile(B)and0.02mol/L potassium dihydrogen phosphate solutions(containing0.2%triethylamine and adjusted to pH3.0with phosphoric acid,C)with linear gradient elution(0min~3.5min,A∶B∶C=6∶13∶18,8.5min,A∶B∶C=6∶30∶64)and the flow rate was1.0ml/min;the detection wavelength was254nm and the column temperature was30℃.Chloramphenicol was used as the internal standard.RESULTS:
The linear ranges were0.04~0.20mg/ml for chlorapheniramine maleate,0.02~0.10mg/ml for furacilin,0.50~2.50mg/ml for ephedrine hydrochlo?ride.The average recoveries were99.44%(RSD=0.48%,n=3)for chlorapheniramine maleate,101.36%(RSD=0.41%,n=3)for furacilin and99.43%(RSD=0.59%,n=3)for ephedrine hydrochloride.CONCLUSION:
The method is reliable,accurate and suitable for quality control of Puma nose drops.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Idioma:
Chinês
Revista:
China Pharmacy
Ano de publicação:
1991
Tipo de documento:
Artigo
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