Expression and significance of platelet membrane glycoproteins and antiplatelet antibody in patients with anaphylactoid purpura / 中华皮肤科杂志

ABSTRACT

Objective To investigate the expression and significance of platelet membrane glyco- proteins and antiplatelet antibody in patients with anaphylactoid purpura.Methods Forty-five patients with anaphylactoid purpura were divided into three groups according to their clinical manifestations,15 in dermal purpura group,18 in mixed group and 12 in nephritis group.The expression of CD62P,CD63 and CD41 in these 45 patients was detected by flow cytometry.The level of antiplatetlet antibody was analysed by ELISA with monoclonal antibody as the probe.Results The percentage of CD62P,CD63 and the level of an- tiplatelet antibody in patients in acute phase were significantly higher than those in regressive phase and those in normal control.Neither of the expression of CD62P,CD63 and CD41,nor the level of antiplatetlet antibody in nephritis group was significantly different from that in the mixed group.Compared with dermal purpura group,the percentage of CD62P was significantly higher in the nephritis group and mixed group. There was no significant difference in CD41 expression among all groups.Conclusion Abnormal humoral immunity exists in anaphylactoid purpura.The activation of platelet could be closely related to the progress of anaphylactoid purpura.CD62P and CD63 could be considered as an index for monitoring of symptoms, determination of prognosis and predicting the outcome in anaphylactoid purpura.