The Influence of Calf Serum from Different Siurces on Lymphicyte Function Study in votri / 中国免疫学杂志

ABSTRACT

Lymphocyte culture media are commonly supplemented With calf serum.The use of calf sera of different sources may introduce uncontrollable variables,which often make trobles in explaining the results.In this papor the influence of calf sera from two different supplies(serum A and B)on lymihocyte funetion assay in vitro was compared.It was found that no difference was observed in B lymohocyte pr- oliferative rosponse to Staphylococcus aureus Cowen strain l(SAC)or to goat F(ab) anti-human ? chain spocific antibody and in plaque-formation cell response induced by SAC or Pokeweed mitogen(PWM)or in PHA induced T cell proliferation in using calf serum A and B.However,in Con?A-driven T cell proliferation,serum B.However, in Con?A-driven T cell proliferation,serum B gave highor cpm m all three experim- ents.The results were 7934?3859?versus 1435?124(p