Search for Gene Expression in Cervical Squamous Cell Carcinoma using GeneFishing(TM) DEG PCR Technique / 대한산부인과학회지
Korean Journal of Obstetrics and Gynecology
; : 617-627, 2005.
Article
em Ko
| WPRIM
| ID: wpr-67468
Biblioteca responsável:
WPRO
ABSTRACT
OBJECTIVE: The aim of this study was to investigate the gene expression profiles using GeneFishing(TM) DEG kit in Korean women with cervical squamous cell carcinoma. METHODS: Cervical cancer biopsies were obtained from patients at the Department of Obstetrics and Gynecology, St. Mary's hodpital. In this study, we used a common reference that was mixed with an equal amount of RNA extracted from non-cervical cancer patients. The profiles of expression genes between cervical normal and squamous cell carcinoma tissue were identified using GeneFishing(TM) DEG Kit and screened by BLAST search. RESULTS: Almost 100 differential expressed genes were identified in universal control and cervical squamous cell carcinoma, 53 of differential expressed genes, up-regulated expression of 32 and 21 down-regulated expression was sequenced. Up-regulated genes were calcylin, calgranulin A, TRK oncogene, HLC5, fibrillarin, collagene type I alpha1 etc. and down-regulated genes were galectin 1, PRP8 pre-mRNA precessing factor 8 homology, clusterin etc. CONCLUSION: We identified gene expression profile in cervical squamous cell carcinoma using GeneFishing(TM) Kit in Korean women. The functional genomics of these genes should be further studied.
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Texto completo:
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Índice:
WPRIM
Assunto principal:
Oncogenes
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Biópsia
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RNA
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Precursores de RNA
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Carcinoma de Células Escamosas
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Expressão Gênica
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Neoplasias do Colo do Útero
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Reação em Cadeia da Polimerase
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Colágeno
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Genômica
Tipo de estudo:
Prognostic_studies
Limite:
Female
/
Humans
Idioma:
Ko
Revista:
Korean Journal of Obstetrics and Gynecology
Ano de publicação:
2005
Tipo de documento:
Article