Committed differentiation of CD34~+ hematopoietic progenitor cells / 中国癌症杂志

ABSTRACT

Purpose:To study the capacity of hematopoietic growth factors combinations(IL 3,G CSF,GM CSF) for inducing CD34 + cells to proliferate and differentiate committedly to granulocytes. Methods:CD34 + cells were isolated from peripheral blood by using a high gradient magnetic cell sorting sysem(MACS),and expanded with IL 3、G CSF and GM CSF in a liquid culture system.Colony forming cells and CD34 + cell ratio were studied by colony forming assays and FACS. Results:The combinations of IL 3,G CSF and GM CSF increased the total cell number by 56 fold and 110 fold, increased CFU GM by 8.44 fold and 35.08 fold respectively at the 3rd and the 7th day after culturing ,and the ratio of CD34 +cell decreased during the culture.Conclusions:It is possible to induce CD34 +cells to proliferate and differentiate committedly to granulocyte lineage.