Purification and functional assay of recombinant human interleukin 18 / 中国免疫学杂志
Chinese Journal of Immunology
;
(12)2000.
Artigo
em Chinês
| WPRIM
| ID: wpr-674870
ABSTRACT
Objective:
To purify recombinant human interleukin 18(rhIL 18) and perform activity assay of renatured rhIL 18Methods:
The rhIL 18 was purified by molecular sieve chromatography MTT assay and RT PCR method were used in activity assay of rhIL 18Results:
The purity of rhIL 18 was up to 96% The renatured rhIL 18 could enhance the cytotoxicity of NK cells and induce IFN ? gene expression in PBMCConclusion:
The rhIL 18 prepared exerts functional activity and can be applied for further studies
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Idioma:
Chinês
Revista:
Chinese Journal of Immunology
Ano de publicação:
2000
Tipo de documento:
Artigo
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