Expression and significance of CD62P in peripheral blood and renal tissue in patients with aged primary nephrotic syndrome / 中国免疫学杂志

ABSTRACT

Objective:To study the changes of CD62P expression in peripheral blood and renal tissue in aged patients with primary nephrotic syndrome(APNS) and to explore its effects on APNS.Methods:Peripheral blood samples and renal tissues of 36 APNS patients were collected.The number of platelets expressing CD62P was counted by flow cytomerty.Renal frozen sections were coated with fluorescent isothiocyanate conjugated monoclonal anti CD62P antibody.Results:Compared with the healthy control subjects,the number of platelets expressing CD62P in APNS patients was significantly higher.CD62P staining in renal tissues was found in 32 of 36 APNS patients,while the staining in renal tissues was negative in 8 control subjects.In APNS patients,the level of CD62P expression of peripheral blood was correlated with that of renal tissue and both of the levels of CD62P expression in peripheral blood and in renal tissue were correlated with 24 h urine protein respectively.Conclusion:The expressions of CD62P in APNS patients increase both in peripheral blood and in renal tissue;CD62P involes in the pathological course of APNS;The levels of CD62P expression of peripheral blood and renal tissue can reflect the condition severity of APNS patients,thus they have important value in clinic.