Expression and implication of Fas and FasL modulate by cytokines in renal cell carcinoma cells / 中国免疫学杂志

ABSTRACT

Objective:To investigate the role of cytokines in modulating expression of Fas and FasL in renal cell carcinoma cells(RCCs) and its implication.Methods:Combination treatment of 786 0 and GRC 1 cells with cytokines including IFN??IFN?,IL 2,TNF? and anti Fas monoclonal antibody (FasAb) to induc apoptosis.FasL function was assessed by coculture assays in vitro using renal cancer cells 786 0 or GRC 1 and Fas sensitive Jurkate T cells.Results:1.Either IFN? or IFN? could up regulate the Fas expression and subsequently augment the Fas mediated apoptosis in 786 0 and GRC 1 cells.2.IFN? and IFN? could up regulate the FasL expression in 780 0 and GRC 1 cells.And subsequently augmente the apoptosis of Jurkat T cells cocultured with 786 0 and GRC 1 cells.IFN? had the same effects on 786 0 cells.IFN? had the same effects on GRC 1 cells.Conclusion:IFN? and IFN? could agument the Fas mediated apoptosis of RCCs by enhancement of Fas expression,but they also up regulate the expression of FasL in RCCs and subsequently augmente the apoptosis of RCCs by enhancment the apoptosis of T lymphocytes by Fas/FasL pathway.