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High level expression of 6His-hEra / 中国免疫学杂志
Chinese Journal of Immunology ; (12)2000.
Artigo em Chinês | WPRIM | ID: wpr-675167
ABSTRACT

Objective:

To study the expression of human Era.

Methods:

The encoding region of human Era gene was inserted into pUC19 plasmid and sequenced,the gene fragment was then cloned into the prokaryotic fusion expression vector pRSET B to contruct recombinant expression plasmid pRSET B hEra.The recombinant protein was expressed in E.coli BL21DE3(plys S).

Results:

6His hEra was expressed after induced by IPTG and took 51.2% of the total cell lysate and mainly existed in the inclusion body.

Conclusion:

High level of human Era was established and this result may shed light on the further study on function of human Era.

Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Idioma: Chinês Revista: Chinese Journal of Immunology Ano de publicação: 2000 Tipo de documento: Artigo

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Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Idioma: Chinês Revista: Chinese Journal of Immunology Ano de publicação: 2000 Tipo de documento: Artigo