DNA mediated immunization co-injected with CpG-oligodeoxynucleotides in hepatitis B virus transgenic mice / 中国免疫学杂志

ABSTRACT

Objective:To study the immune responses of HBV based immunization co injecting CpG oligodeoxynucleotides in HBV transgenic mice.Methods:The HBV transgenic mice were immunized by multiple sites intramuscular injection with V HBs and CpG ODN or V HBs alone or V 1012 controls.The anti HBs Ab and HBsAg in serum from immunized mice was measured by ELISA.The expression of HBsAg in liver tissue was detected by immunohistochemistry method(SP).The histological activity index was calculated with Knodell's method.Results:The anti HBs Ab in serum could be detected and HBsAg in serum and liver tissues could not be measured in 2 out of 6 HBV transgenic mice immunized with V HBs+CpG ODN.There were not significantly changes in the level of anti HBs Ab and HBsAg in serum from HBV transgenic mice immunized with V HBs or V 1012 alone.The histological activity indexes in liver tissues from mice immunized with V HBs+CpG ODN were higher than that in mice inoculated with V HBs or V 1012 alone.The large number of lymphocytes could be found in liver tissues in V HBs+CpG ODN immunization group by microscopy.Conclusion:The immuno response,which seems to be responsible for the disappearance of HBsAg,might be elicited in HBV transgenic mice immunize with V HBs+CpG ODN.