Experimental study of AADC gene therapy for Parkinson’s disease by direct intracerebral gene transfer mediated by cationic liposome / 中华神经科杂志

ABSTRACT

Objective To study the roles of AADC gene therapy for Parkinson’s disease and its action by coadministrating exogenous L dopa and discuss the effectiveness by way of cationic liposome mediated gene transferring into the brain Methods Injecting either the pCDNA3 AADC Liposome or pCDNA3 Liposome complexes into the striatum of Parkinsonian SD rats in experimental and control groups respectively, observing their improvements of rotational behavior, and furthermore, observing the improvement after combining with administration of exogenous L dopa were carried out The expression of AADC were assayed by using immunohistochemical staining Results (1) Intracerebral transplantation of pCDNA3 AADC Liposome complex improved the rotational behavior of Parkinsonian rats in some degree when its being observed at 3,7,14,21 and 28 days( P 0 05), the maximum improvement was at the time of 1 week (2) After coadministration of exogenous L dopa (10 mg/kg weight) per days, the experimental groups reached much more obvious improvement than that in the controls at the same time described above( P 0 05) (3) Immunohistochemical staining showed that the AADC were expressed stably in the striatum Conclusions Increase of AADC gene expression in the brain may improve the rotational behavior of Parkinsonian rats as well as elevate the curative effect of exogenous L dopa therapy, suggesting that it should be helpful to maintain a long term L dopa theraputic effect at a much lower level and avoid all kinds of side effects The technic of intracerebral gene transfer mediated by cationic liposome might be a new method for gene therapy of PD