The Effects XGD-1 on the Proliferation of Human Peripheral Blood T Lymphocyte and the Expression of IL-2R / 中国普外基础与临床杂志

ABSTRACT

Objective To explore the immunosuppressive effect of XGD 1 and its mechanism. Methods Different concentrations of XGD 1 were added to PHA or ConA induced human peripheral blood T lymphocyte.Seventy two hours later modified MTT assay was employed to test the effect of XGD 1 on T cell proliferation. Flowcytometry was used to examine the effect of XGD 1 on the expression of IL 2 receptor(IL 2R) on the surface of T cells individually at 48 h and 72 h.And the effects of XGD 1 combined with cyclosporine A(CsA) on the proliferation of T lymphocytes and the expression of IL 2R were also investigated. Results In the concentration range of 0.2～25 ?g/ml,XGD 1 exerted marked inhibitory effect on PHA or ConA induced T cell proliferation,which was proportional to dose. Flowcytometry showed that XGD 1 inhibited the expression of IL 2R,and the percentage of IL 2R? positive cells after stimulation of PHA decreased from 47.67% to 25.03% in the presence of XGD 1 (1 ?g/ml).XGD 1 and CsA had synergism in inhibition of T cell proliferation and IL 2R expression. Conclusion The study suggests that XGD 1 has immunosuppressive effect. The suppressive effect of XGD 1 on T cell proliferation is most probably mediated by decreasing IL 2R expression.