Suppression of Mdr1 gene using RNA interference in cultured rat astrocytes induced by coriaria lactone / 中华神经科杂志
Chinese Journal of Neurology
; (12)2001.
Article
em Zh
| WPRIM
| ID: wpr-676280
Biblioteca responsável:
WPRO
ABSTRACT
Objective To reverse P-glycoprotein-mediated muhiple drug resistance using RNA interference(RNAi)in cultured rat astrocytes.Methods Astroeytes overexpressing P-glyeoprotein induced by coriaria lactone were transfected with the short-hairpin RNA expression vector-p-SIREN shuttle designed to target Mdrl mRNA.The mRNA level of Mdrl gene was evaluated by real-time PCR;the P-glycoprotein was examined by immunocytochemistry and image analysis,meanwhile the rhodamine efflux was assessed by flow cytometry.Results The astrocyte model overexpressing P-glycoprotein were established and successfully transfected with the short-hairpin RNA expression vector-p-SIREN shuttle.The mRNA level of Mdrl gene was knocked down by 67.70%(P
Texto completo:
1
Índice:
WPRIM
Idioma:
Zh
Revista:
Chinese Journal of Neurology
Ano de publicação:
2001
Tipo de documento:
Article