Determination of homoharringtonine distribution in eyeball tissue ofrabbit by high performance liquid chromatography method / 中国临床药理学与治疗学

ABSTRACT

Aim To observe homoharringtonine(HHT) distribution in eyeball tissue of rabbit. Methods Harringtonine(HT) was used as internal standard. The chromatography consisted of Kromasil C18 column with methanol 0.01mol?L-1 (60∶40,pH 2.5) as the mobile phase at the flow rate of 0.8ml?min-1. Results The chromatography retention time of HHT and HT were 6.35min and 4.70min. The standard curve was linear in the concentration range of 5～200 ?g?L-1 in serum and the relation coefficient was 0.994. The recovery of spiked sample was(93.17?4.31)%. After injection of 2mg of HHT in side of rabbit eyeball, the contents in sclera, cornea, retina, vitreous body and crystalline lens were reduced progressively, but the eliminate rate were reduced in a proper order. Conclusion HHT can distribute to every eyeball tissue when it was injected in side of rabbit eyeball.