Effect of taurine on changes of nuclear and cytosolic [Ca~(2+) ] elicited by H_2O_2 in rat vascular smooth muscle cells / 中国药理学通报

ABSTRACT

AIM As one of the reactive oxygen species, toxic dose of H 2O 2 leads to the necrosis or apoptosis of many kinds of cells. It is nuclear whether these processes are related with the changes of nuclear Ca 2+ elicited by H 2O 2. This study on the effect of taurine on changes of nuclear and cytosolic (Ca 2+ ) elicited by H 2O 2 in rat vascular smooth muscle cells. METHODS The techniques of Fluo 3 and laser scanning confocal microscopy were used. RESULTS Rapid and sustaining rise of nuclear [Ca 2+ ] ([Ca 2+ ] n) and cytosolic [Ca 2+ ]([Ca 2+ ] c) were found in cultured rat vascular smooth muscle cells upon adding of 0 5% H 2O 2. Pretreatment of the cells with 20 mmol?L -1 taurine, a regulator of cellular Ca 2+ homestasis, significantly decreased the rise amplitude of [Ca 2+ ] n ( P 0 05) elicited by H 2O 2. It was suggested that the changes of [Ca 2+ ] n and [Ca 2+ ] c have different respond to taurine. CONCLUSION It was concluded that there exist the independent regulating mechanisms of Ca 2+ in the nucleus from differences between the nucleus and cytosol Ca 2+ in sensitivity to action of H 2O 2 and taurine.