High performance liquid chromatography analysis of porcine platelet derived growth factor and its effect on DNA synthesis of human vascular endothelial cells / 第二军医大学学报

ABSTRACT

Objective: To determine the molecular weight and purity of porcine platelet derived growth factor (pPDGF) and to investigate its effect on DNA synthesis of human umbilical vein endothelial cells. Methods: In the present experiment, the high performance liquid chromatography was used and the molecular weight and purity of pPDGF were studied. Human umbilical vein endothelial cells was cultured and effects of pPDGF on DNA synthesis of endothelial cells was observed by 3H TdR incorporation in vitro . Results: The findings of high performance liquid chromatography showed that the molecular weight of pPDGF was 29 120 and the purity was 89.46%, and pPDGF significantly promoted DNA synthesis of quiescent endothelial cells with a maximal response at a concentration of 40 ng/ml at 48 h. Conclusion: The molecular weight of pPDGF is 29 120, and it can promote DNA synthesis of cultured human umbilical vein endothelial cells. [