EFFECT OF VITAMIN A DEFICIENCY ON LIPID PEROXIDATION AND ANTIOXIDATION SYSTEM IN RATS / 营养学报

ABSTRACT

Objective: To study the effect of vitamin A deficiency on lipid peroxidation and antioxidation system in rats. [WT5FZ]Methods: According to body weight, 33 Wistar male rats were randomly divided into 3 groups, A (Vitamin A complete deficiency group), B (Vitamin A low grade deficiency group), C (Vitamin A normal control group). The study lasted 84 days. The serum vitamin A, serum, liver and brain SOD activities, blood, liver and brain GSH Px activities, serum, liver and brain MDA were studied. Spin trapping techniques combined with ESR were used to capture and detect free radicals generated in liver. [WT5FZ]Results: In vitamin A completely deficient group and vitamin A low grade deficient group, SOD activities of serum, liver and brain, and the GSH Px activities of blood, liver and brain decreased significantly, while LPO contents of serum, liver and serum increased markedly, and typical free radicals peaks in liver were indicated by ESR spectra. [WT5FZ]Conclusion: Vitamin A deficiency results in decrease of antioxidation capacity and increase of lipid peroxidation.