Screening out CpG immunostimulatory sequence-specific DNA-binding proteins with Yeast-one-hybrid system / 第二军医大学学报
Academic Journal of Second Military Medical University
;
(12)1985.
Artigo
em Chinês
| WPRIM
| ID: wpr-677409
ABSTRACT
Objective:
To screen out DNA binding proteins specifically recognizing CpG immunostimulatory sequence (ISS) for further investigating the molecular mechanisms of ISS.Methods:
Yeast one hybrid system was adapted in screening a human bone marrow cDNA library using 4 copies of ISS as bait. The ISS binding activity of the positive clone was confirmed by electrophoretic mobility shift assay (EMSA).Results:
Four dual positive colonies were obtained, two of them encoded proteins with unknown functions. The other 2 encoded light chains of immunoglobulin with amino sequences homology to anti DNA Ab and HBsAb respectively. EMSA showed HBsAb specifically bound to CpG ODN at pH6.4 and pH 5.8.Conclusion:
HBsAb may have ISS specific DNA binding activity.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Tipo de estudo:
Estudo diagnóstico
/
Estudo de rastreamento
Idioma:
Chinês
Revista:
Academic Journal of Second Military Medical University
Ano de publicação:
1985
Tipo de documento:
Artigo
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