EFFECT OF LYCIUM BARBARUM POLYSACCHARIDE ON APOPTOSIS OF HUMAN LEUKEMIA CELLS / 营养学报
Acta Nutrimenta Sinica
;
(6)1956.
Artigo
em Chinês
| WPRIM
| ID: wpr-677495
ABSTRACT
Objective:
To explore the effect of LBP X on apoptosis of human leukemia HL 60 cells.Methods:
The inhibitory effect of LBP X on growth of HL 60 cells was assayed by MTT method. The membrance fludity was determined with DPH fluorigenic labeling technique. HL 60 cells were stained with Hoechst 33 258 for nuclear morphology analysis. Agarose gel electrophoresis and flow cytometry were used to analyze apoptosis qualitatively and quantitatively.Results:
20-1 000mg/L LBP X inhibited the growth of HL 60 cells in dose dependent manner and the membrance fludity decreased. The nuclei of HL 60 cells treated with LBP X for 48 h shrinked, condensed and cleaved. Agarose gel electrophoresis of DNA from cells treated with LBP X revealed "DNA ladder". HL 60 cells exposed to LBP X showed apoptotic peaks.Conclusion:
LBP X can induce apoptosis in human leukemia HL 60 cells.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Idioma:
Chinês
Revista:
Acta Nutrimenta Sinica
Ano de publicação:
1956
Tipo de documento:
Artigo
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