EFFECTS OF IL-8 AND IFN-? ON CULTURED ECTOPIC ENDOMETRIAL CELLS / 解放军医学杂志
Medical Journal of Chinese People's Liberation Army
;
(12)2001.
Artigo
em Chinês
| WPRIM
| ID: wpr-678626
ABSTRACT
Objective The purpose of this study was to investigate the effects of interleukin 8 (IL 8) and interferon (IFN) alpha on proliferation of ectopic endometrial cells in vitro . Methods Ectopic endometrial cells from women suffering from endometriosis were cultured, purified, and identified by immunocytochemistry. Cultured endometrial cells were treated with IL 8 and IFN ? 2b in various concentrations in 96 well plates (IL 8 in 0 1ng/ml, 1ng/ml, 5ng/ml, 10ng/ml, 50ng/ml, IFN ? in 10U/ml, 50U/ml, 100U/ml, 1 000U/ml, 10 000U/ml). The effects of IL 8 and IFN ? 2b on endometrial cell proliferation were assessed using MTT. The ultrastructural changes in endometrial cells, which were incubated in medium containing 1ng/ml of IL 8, were observed with transmission electron microscopy. Results The data suggested that there was a dose dependent stimulatory effect of IL 8 on cell proliferation. IL 8 could stimulate proliferation of endometrial cells, and the most effective concentration was 10ng/ml ( P
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Idioma:
Chinês
Revista:
Medical Journal of Chinese People's Liberation Army
Ano de publicação:
2001
Tipo de documento:
Artigo
Similares
MEDLINE
...
LILACS
LIS