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Culture of osteoblasts by serial explant culture and comparison of their characteristics / 北京大学学报(医学版)
Journal of Peking University(Health Sciences) ; (6)2004.
Artigo em Chinês | WPRIM | ID: wpr-678755
ABSTRACT

Objective:

To Investigate a simple, economical and efficient method of primary osteoblast culture and compare their characteristics.

Methods:

Primary osteoblasts of 1 st -4 th series explant culture from calvarial bones of neonatal Sprague Dawley rats,were collected and osteoblasts shape, mitosis, proliferation, ALP activity and immunohistological expression of osteocalcin and BMP 2 observed.

Results:

No difference was found in cell shape, spread, conflunce, ALP activity as well as immunohistological stain of osteocalcin and BMP 2 of the osteoblasts, which had been harvested from the 1 st -4 th series explant cultures of Sprague Dawley rats.

Conclusion:

Series explant culture can harvest the same kind of osteoblasts as the explant culture, and more osteoblasts can be obtained at a single time. It saves money and time and is easy to manipulate.

Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Idioma: Chinês Revista: Journal of Peking University(Health Sciences) Ano de publicação: 2004 Tipo de documento: Artigo

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Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Idioma: Chinês Revista: Journal of Peking University(Health Sciences) Ano de publicação: 2004 Tipo de documento: Artigo