Observation of the morphology of cross-linked starch iodine and its virucidal efficacy / 第三军医大学学报

ABSTRACT

Objective To detect the virucidal efficacy of cross linked starch iodine (CSI) and its influence factors in solution and to observe the morphological changes of CSI after disinfection. Methods Sindbis virus (Sindbis) and poliovirus (polio) were used as the test viruses, and different concentrations of plasm as the organic solvent. The virucidal efficacy of CSI was observed by means of cell infection test. The morphological changes of CSI particles after disinfection were observed by scanning electron microscopy. Results CSI had the same virucidal effect on Sindbis and polio viruses in solution. After treatment with CSI at a dose of 50-100 mg/ml for 30-60 min, the viruses were inactivated by 5-7 logs TCID 50 . The virucidal efficacy of CSI enhanced in dose and time dependent manners. CSI could inactivate the viruses in D hanks solution with 10% plasma or in plasma by 2-5 logs TCID 50 and 2-3 logs TCID 50 , respectively. Electron microscopy showed that the CSI was in the form of round and oval particles with the diameter of about 100 ?m. After disinfection, the particle surface became more lubricious, but no morphological changes were observed. Conclusion CSI has effective virucidal effect on envelope viruses and non envelope viruses in solution free of organic substances, but the virucidal efficacy can be greatly affected by organic substances. There is no morphological change of CSI particles after disinfection.